maize reference genome b73 refgen-v4  (Refgen Technologies INC)

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Analysis of ZmLPA1 gene in maize. ( A ) Alignment of ZmLPA1 gene cDNA sequences in B73 and lpa1 , amino acid changes are marked with red box; ( B ) Phylogenetic tree of ZmLPA1 gene; ( C ) Transcription expression of ZmLPA1 gene in different tissues of maize.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Analysis of relevant characters of wild-type B73 and mutant lpa1 cloverleaf.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Investigation of agronomic characters of wild-type B73 and mutant lpa1 .

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Effects of exogenous hormones on leaf angle of wild-type B73 and mutant lpa1 . ( A ) Effects of different concentrations of IAA on leaf angle of wild type and mutant. ( B ) Size of leaf angle for wild type and mutant under different concentrations of IAA treatment.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Upset plot analysis of DEGs in B73 and lpa1 under different treatments. The columns indicate DEGs under single or multiple processes, the x -axis represents combinations of different groups (black dots represent single-treatment comparisons, black lines connected by dots represent multiple-treatment comparisons) and the y -axis represents the number of genes corresponding to the combinations.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: GO annotations of DEGs in B73 and lpa1 identified under different treatments. ( A ) B73-CK vs. B73-100; ( B ) B73-CK vs. B73-500; ( C ) lpa1-CK vs. lpa1-100; ( D ) lpa1-CK vs. lpa1-500. CK = control, 100 = 100 μmol·L −1 IAA, and 500 = 500 μmol·L −1 IAA.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Pathway enrichment analysis of B73 and lpa1 exposed to different treatments. ( A ) B73-CK vs. B73-100; ( B ) B73-CK vs. B73-500; ( C ) lpa1 -CK vs. lpa1 -100; ( D ) lpa1 -CK vs. lpa1 -500. CK = control, 100 = 100 μmol·L −1 IAA, and 500 = 500 μmol·L −1 IAA.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: Analysis of transcription factors differentially expressed in B73 and lpa1 under different treatments.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: The expression pattern of eight selected genes identified by RNA-seq was verified by qRT-PCR. ( A ) Heat map showing the expression changes (log 2 fold change) in response to the B73-CK to lpa1 -500 treatments for each candidate gene as measured by RNA-seq and qRT-PCR; ( B ) Scatter plot showing the changes in the expression (log-fold change) of selected genes based on RNA-seq via qRT-PCR. The gene expression levels are indicated by colored bars.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Expressing, RNA Sequencing, Quantitative RT-PCR, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway

doi: 10.3390/ijms23094886

Figure Lengend Snippet: ZmLPA1 gene regulates maize leaf angle. ZmLPA1 acts as a central gene that directly regulates the auxin signaling pathway, thereby regulating the size of leaf angle. The red circle indicates that the expression of auxin-related genes in mutant lpa1 is higher than that in wild-type B73 under exogenous IAA treatment.

Article Snippet: Comparison of clean reads was obtained from the screen to the B73 RefGen v4 maize reference genome sequence using HISAT software.

Techniques: Expressing, Mutagenesis